Dna Sequences Using Polymerase Chain Reaction - 1605 Words

Amplification of 16S Ribosomal DNA Sequences using Polymerase Chain Reaction Edwina Abou Haidar, Houssam Al Koussa, Mary AbedAlAhad. Department of Biology, Lebanese American University, Byblos, Lebanon Abstract The 16s rRNA gene sequencing is a widely common amplicon sequencing method used to identify and compare bacteria in a given sample. This method is well established and allows to study phylogeny and taxonomy of complex microbiomes. In this study, an unknown sample of extracted microbial DNA was analyzed by performing the polymerase chain reaction followed by agarose gel electrophoresis. The results were accurate since three distinct bands (1500 basepairs) corresponded to our sample (duplicates) and the positive control. This indicates clearly the amplification of the 16s rRNA gene whose further sequencing technique constitutes a pivotal tool in the accurate identification of bacterial isolates as well as the discovery of novel bacteria in clinical microbiology laboratories. Abbreviations rDNA: ribosomal DNA rRNA: ribosomal RNA bp: base pair PCR: Polymerase Chain Reaction Introduction Physiological and biochemical tests constituting the basis of conventional differentiation between bacterial species are somehow cumbersome, consume a lot of time and require different approaches [1]. Furthermore, the commercial identification systems failed to identify commonly encountered bacteria and uncommon isolates. In fact, these commercial systemsShow MoreRelatedForensic Science : An Effective Tool For The Law Enforcement1319 Words   |  6 Pagesways to do so come from the effectiveness of DNA analysis. Forensic scientist play a vital role in helping catch criminals, using different devices so as DNA markers and DNA testing along with many more different types to help catch a criminal. It is possible for DNA analysis to have unfounded results that can lead to an unjust conviction, with most of the time, resulting from human error. Although there remains diverse thoughts on the issues of DNA analysis being the most effective too l for theRead MoreAbstract. Taq Polymerase Is Essential In Polymerase Chain1446 Words   |  6 Pages ABSTRACT Taq Polymerase is essential in Polymerase Chain Reaction(PCR) experiments to obtain a PCR amplification of an unknown gene. The unknown gene is then ligated into a vector plasmid, which is placed in a bacterium Escherichia Coli to transform and multiply. Ultimately, identification and characterization of the unknown gene is done using electrophoreses and gel imaging. Cloning techniques such as the one performed have been used for many years to isolate genes from a variety of species.Read MorePolymerase Chain Reaction Essay1003 Words   |  5 Pagesgenetic molecular level, where a piece of DNA is copied on a large-scale by genetically copying tens to hundreds of thousands of identical DNA fragments. Researchers are developing new methods of cloning by using polymerase chain reaction (PCR). PCR was introduced in the 1980s and in recent years Kary Mullis won the Nobel Prize in Chemistry for his invention of PCR. Today, Scientists today are researching the various sub-fields of cloning , using PCR, in new ways using terminators, enzyme insertion, andRead MoreDna Barcoding Using Coi For Species Identification For Conservation1573 Words   |  7 PagesDNA Barcoding using COI for Species Identification for Conservation Introduction and Literature Review The DNA Barcoding is becoming more popular in the present times due to its accuracy in the identification of different species. It has been approved to be more accurate than other taxonomic methods. The DNA Barcoding employs in the use of Polymerase Chain Reaction to magnify the COI gene. The amplified COI genes of the organisms are sequenced and compared with a known database of the organismsRead MoreOxidatic Polymorphism In Oral Cancer Lab Report884 Words   |  4 Pagesantioxidant enzymes and the thiol were assayed and analysed by using spectrophotometric methods. Gene GSTT1 was analysed by using polymerase chain reaction(PCR). The some biochemical technique was used for the study of oxidative stress and the genetic polymorphism of the antioxidant enzymes level or status in patient with oral cancer. The main Aim is study of genetic polymorphism in the patients suffering with oral cancer and also the isolation of DNA, for this various methods are used. Genetic polymorphism-Read MorePcr Rflp Report : Pcr1166 Words   |  5 PagesPCR-RFLP Report PCR What is it: The Polymerase Chain Reaction is a method that uses the capability of DNA polymerase to synthesize to new DNA strands which are matching to the template strand. A primer needs to be added to the first nucleotide due to the fact that DNA polymerase only can add a nucleotide only onto a 3 -OH group that already exists. Because of this condition, we are able to define a chosen region of template sequence which we can then generate millions to billions of copies. ThisRead MoreThe Community of Microorganisms that Reside in the Epithelia of Humans1034 Words   |  5 PagesThis information could only be acquired through processes such as DNA extraction, amplification of specific genetic target by the polymerase chain reaction (PCR), agarose gel electrophoresis, restriction enzyme digestion, cloning of DNA fragments into plasmid vector, transformation and blue/white clone colony screening. Through the phylogenetic tree analysis Introduction This lab consisted in the amplification of 16s rRNA gene sequence, PCR based sequencing of forearm bacterial components. The 16s geneRead MoreScience947 Words   |  4 PagesDNA Technology Task 3 – Poster DNA fingerprinting, also known as DNA profiling, is a technique used in forensic science that identifies individuals based on various characteristics of their DNA. Although the DNA sequences between humans are 99.9% identical, DNA fingerprinting is able to distinguish between individuals due to the presence of specific sequences within the non-coding region of the genome known as satellite DNA. This satellite DNA consists of long stretches of DNA made up of repeatingRead MoreA Segment Of The Will Die Slowly Gene From Drosophila Melanogaster1688 Words   |  7 PagesTA cloning, sequencing and sequence analysis of a segment of the will die slowly gene from Drosophila melanogaster.† Abstact: A seemingly novel gene sequence has been discovered in fruit flies from Nicholas Harden’s lab at Simon Fraser University using random PCR primers. Attempts at purifying and characterizing this gene have been elusive. Here, we show methods for isolating, amplifying, and purifying the gene of interest for analysis. Using polymerase chain reaction to amplify the gene, it isRead MorePolymerase Chain Reaction ( Pcr )1564 Words   |  7 PagesMBB/BIO 181 | Polymerase Chain Reaction (PCR) Name: Teresa Naval INTRODUCTION Scientifically, why is the study of Alu insertions interesting? (10 pts) Alu insertions are biological tools that can trace human history, evolution, and migration. In particular, Alu is often used to understand prehistory because all primates (including humans) with Alu insertions at specific locations can be traced to common ancestors [3]. Alu is sometimes referred to as a â€Å"jumping gene† because it copies itself